A Defined Subset of Adenylyl Cyclases Is Regulated by Bicarbonate Ion
نویسندگان
چکیده
منابع مشابه
A defined subset of adenylyl cyclases is regulated by bicarbonate ion.
The molecular basis by which organisms detect and respond to fluctuations in inorganic carbon is not known. The cyaB1 gene of the cyanobacterium Anabaena sp. PCC7120 codes for a multidomain protein with a C-terminal class III adenylyl cyclase catalyst that was specifically stimulated by bicarbonate ion (EC50 9.6 mm). Bicarbonate lowered substrate affinity but increased reaction velocity. A poin...
متن کاملBicarbonate-regulated soluble adenylyl cyclase.
Soluble adenylyl cyclase (sAC) represents a novel form of mammalian adenylyl cyclase structurally, molecularly, and biochemically distinct from the G protein-regulated, transmembrane adenylyl cyclases (tmACs). sAC possesses no transmembrane domains and is insensitive to classic modulators of tmACs, such as heterotrimeric G proteins and P site ligands. Thus, sAC defines an independently regulate...
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The Slr1991 adenylyl cyclase of the model prokaroyte Synechocystis PCC 6803 was stimulated 2-fold at 20 mM total C(i) (inorganic carbon) at pH 7.5 through an increase in k(cat). A dose response demonstrated an EC50 of 52.7 mM total C(i) at pH 6.5. Slr1991 adenylyl cyclase was activated by CO2, but not by HCO3-. CO2 regulation of adenylyl cyclase was conserved in the CyaB1 adenylyl cyclase of An...
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Soluble adenylyl cyclase (sAC) is a source of the second messenger cyclic adenosine 3', 5' monophosphate (cAMP). sAC is directly regulated by bicarbonate (HCO(-) 3) ions. In living cells, HCO(-) 3 ions are in nearly instantaneous equilibrium with carbon dioxide (CO2) and pH due to the ubiquitous presence of carbonic anhydrases. Numerous biological processes are regulated by CO2, HCO(-) 3, and/o...
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14. Hrandt, H. I,., Hagiwara, S., Kidokoro, Y. and Miyazaki, S. (1976) J. Physiol. (London) 263, 4 17-439 Grohovaz, F., Zacchetti, I)., Clementi, E., Lorenzon, P., Meldolesi, J. and Fumagalli, G. (1991) J. Cell Hiol. 133, 1831-1350 Kidokoro, Y. and Kitchie, A. K. (1980) J. Physiol. (London) 307, 199-216 Fenwick, E. M., Marty, A. and Neher, E. (1982) J. Physiol. (London) 331, 599-635 Hallesta, J...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m303025200